pFucci-S/G2/M Green-Hyg (Expression vector)

MBL

Catalog No.:
MBL-AM-V9010M
pFucci-S/G2/M Green-Hyg (Expression vector)
pFucci-S/G2/M Green-Hyg (Expression vector)
pFucci-S/G2/M Green-Hyg (Expression vector)
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FUCCI (Fluorescent Ubiquitination-based Cell Cycle Indicator) is a set of fluorescent probes which enables the visualization of cell cycle progression in living cells. FUCCI utilizes the phase-dependent nature of replication licensing factors Cdt1 and Geminin. A fusion protein of a fragment of Cdt1 (amino acids 30-120) with the fluorescent protein monomeric Kusabira-Orange 2 (mKO2) serves as an indicator of G1 phase. A fusion protein of a fragment of Geminin (amino acids 1-110 or 1-60) with the fluorescent protein monomeric Azami-Green 1 (mAG1) visualizes the S,G2 and M phase. In summary, FUCCI utilizes the highly selective, rapid degradation of the replication licensing factors mediated by the ubiquitin proteasome system to give excellent visualizations of the cell cycle.

Product Specifications
Formulation Dry form. Reconstitute with distilled water or TE before use.
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